Transmission of linezolid-resistant Enterococcus isolates carrying optrA and poxtA genes in slaughterhouses

Introduction The presence of linezolid-resistant enterococci found in animal-derived food has attracted attention for possible transmission to human-derived through the chain. Linezolid-resistant farms have been widely reported, but carrying antimicrobial resistance (AMR) genes poxtA , optrA or cfr (D) slaughterhouse environments not well addressed. Methods Enterococcus was isolated from samples collected two slaughterhouses Hangzhou, and linezolidin-resistant identified by PCR. minimum inhibitory concentration (MIC) determined microbroth dilution method. Finally, whole genome strains more linezoline sequenced using Oxford Nanopore Technology Results Here, 291 were 309 (94.17%). A total 4 -positive 42 based on susceptibility test showed that highest rate florfenicol 97.82% multidrug (MDR) 95.65%. Two carried multiple linezolid genes, among which casseliflavus CQFYY22-063 cocarrying duck cecum, faecium CQFYH22-006 sewage first time. Furthermore, revealed gene strain located Inc18-type plasmid pFYY063-optrA-70K, pFYY063- poxtA-12K. Meanwhile, Rep3-type pFYH006-poxtA-25K, chromosome. Discussion Together, deserves extensive attention, indicating need strengthen monitoring different links production chain within One Health concept.